Part:BBa_M36051:Design
LexA regulated promoter
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence includes the non coding information prior to the coding sequence of the lexA gene. We did not include the predicted ribosome binding site b/c that is included in the 5' UTR that can be used in a composite part. We know exactly were the lexA protein binds, but we are not entirely sure of the location of the promoter region itself. Because we do not know the exact sequence of the promoter, superfluous information may be included.
Source
escherichia coli k -12
References
Analysis of Escherichia coli RecA Interactions with LexA, CI, and UmuD by Site-Directed Mutagenesis of recA JULIE A. MUSTARD1 and JOHN W. LITTLE
Control of recA Gene RNA in E. coli: Regulatory and Signal Genes Ann McPartland, Linda Green and Harrison Echo
DNA binding properties of the LexA repressor M Schnarr, P Oertel-Buchheit, M Kazmaier, M Granger-Schnarr
Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria Nir Friedman1, Shuki Vardi1[, Michal Ronen, Uri Alon, Joel Stavans
The bacterial LexA transcriptional repressor M. Butalaa, D. Zgur-Bertokb and S. J. W. Busbya
Assigning numbers to the arrows: Parameterizing a gene regulation network by using accurate expression kinetics Michal Ronen, Revital Rosenberg, Boris I. Shraiman, and Uri Alon
Structure of the LexA–DNA complex and implications for SOS box measurement Adrianna P. P. Zhang, Ying Z. Pigli & Phoebe A. Rice