Regulatory

Part:BBa_M36051:Design

Designed by: Ryan Kent   Group: Stanford BIOE44 - S11   (2011-05-02)

LexA regulated promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence includes the non coding information prior to the coding sequence of the lexA gene. We did not include the predicted ribosome binding site b/c that is included in the 5' UTR that can be used in a composite part. We know exactly were the lexA protein binds, but we are not entirely sure of the location of the promoter region itself. Because we do not know the exact sequence of the promoter, superfluous information may be included.

Source

escherichia coli k -12

References

Analysis of Escherichia coli RecA Interactions with LexA, CI, and UmuD by Site-Directed Mutagenesis of recA JULIE A. MUSTARD1 and JOHN W. LITTLE

Control of recA Gene RNA in E. coli: Regulatory and Signal Genes Ann McPartland, Linda Green and Harrison Echo

DNA binding properties of the LexA repressor M Schnarr, P Oertel-Buchheit, M Kazmaier, M Granger-Schnarr

Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria Nir Friedman1, Shuki Vardi1[, Michal Ronen, Uri Alon, Joel Stavans

The bacterial LexA transcriptional repressor M. Butalaa, D. Zgur-Bertokb and S. J. W. Busbya

Assigning numbers to the arrows: Parameterizing a gene regulation network by using accurate expression kinetics Michal Ronen, Revital Rosenberg, Boris I. Shraiman, and Uri Alon

Structure of the LexA–DNA complex and implications for SOS box measurement Adrianna P. P. Zhang, Ying Z. Pigli & Phoebe A. Rice